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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in selling cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually explained. Two supplemental splice variants have been noted but in-depth nucleotide sequence data isn't still obtainable.
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Blocking peptides are peptides that bind precisely into the focus on antibody and block antibody binding. These peptide normally is made up of the epitope regarded through the antibody.
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This information outlines more possible optimization of mobile and immunoassay parameters to guarantee the absolute best click here outcomes are...
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This manual outlines more achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining from the blocked antibody versus the antibody by itself, you can see which staining is distinct.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E sorts a complex with and capabilities to be a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S changeover. This protein accumulates within the G1-S period boundary and it is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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